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Home Antibody All anti-CD14 antibodies

Anti-CD14 Antibody EPR3653

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307685
  • Rabbit monoclonal antibody against CD14 (clone EPR3653 )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC424624) , 20ug
100ul 325 In Stock
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WB(1)
IHC(2)
Assay(1)
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Also for CD14 (NM_000591)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human CD14 was used as an immunogen.
Clone NameEPR3653 IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsWB: 1:1000 - 1:5000; IHC-P: 1:500 - 1:1000; ICC: 1:100 - 1:250
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.
Purification pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens CD14 molecule (CD14), transcript variant 1
Alternative NameCD14 antigen; monocyte differentiation antigen CD14; CD14 molecule
Database LinkNP_000582
FunctionCD14 is a glycophosphatidylinositol-anchored protein expressed on the surfaces of monocytes and plymorphonuclear leukocytes. It acts as a co-receptor for the detection of bacterial lipopolysaccharide (LPS) (1). CD14 cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial LPS. It acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (2). CD14 is expressed strongly on peripheral blood monocytes and macrophages, and weakly on the surface of granulocytes (1).
Related Pathway
Hematopoietic cell lineage
MAPK signaling pathway
Toll-like receptor signaling pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-CD14 antibody [EPR3653]; All lanes : Anti-CD14 antibody [EPR3653] at 1/1000 dilution.Lane 1 : PBMC cell lysate.Lane 2 : Human tonsil cell lysate.Lane 3 : SW480 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat Anti-rabbit HRP at 1/2000 dilution.Predicted band size : 40 kDa.Observed band size : 53 kDa .
Assay Image
Other-Anti-CD14 antibody [EPR3653](TA307685); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653]; Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human placenta tissue labelling CD14 with TA307685 at 1/500 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [EPR3653]; Immunohistochemical analysis of Formalin-fixed, paraffin- embedded Human tonsil tissue labelling CD14 with TA307685 at 1/500 dilution.

 

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