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Anti-DNMT1 Antibody EPR3522
Also for DNMT1 (NM_001130823)
|A synthetic peptide corresponding to residues at the C-terminus of human Dnmt1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||FC: 1:50, ICC: 1:50 - 100, IP: 1:20, WB: 1:1,000 - 10,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1|
|ADCADN; AIM; CXXC9; DNMT; HSN1E; MCMT|
|Dnmt1 is one of three families of enzymes (Dnmt1, Dnmt2, Dnmt3 (a and b)) that have a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Abnormal methylation patterns are associated with certain human tumors and developmental abnormalities (1). Dnmt1 is responsible for maintenance of the DNA methylation pattern after DNA replication. Dnmt1 is a large protein and can be subdivided into an N- and a C-terminal part. The C-terminal domain harbours the catalytic center. The N-terminal part of the enzyme comprises several individual domains, and plays a role in intracellular targeting and regulation of the enzyme’s activity. Dnmt1 shows a preference for hemimethylated CG sites and the specificity of Dnmt1 for hemimethylated target sites is a key property for that function (2). |
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Western blot - Dnmt1 antibody [EPR3522]; All lanes : Anti-Dnmt1 antibody [EPR3522] at 1/1000 dilution.Lane 1 : HuT-78 cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 183 kDa.
Immunocytochemistry/ Immunofluorescence - Dnmt1 antibody [EPR3522]; Immunofluorescent staining of HeLa cells using ab92314