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Anti-BDNF Antibody EP1293
Also for BDNF (NM_001709)
|A synthetic peptide corresponding to residues in human BDNF was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000 - 1:10000; ICC/IF: 1:100 - 1:250; IHC-Fr: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens brain-derived neurotrophic factor (BDNF), transcript variant 4|
|Brain-derived neurotrophic factor (BDNF), like other neurotrophins, is a polypeptide factor initially regarded to be responsible for neuron proliferation, differentiation and survival through its uptake at nerve terminals and retrograde transport to the cell body (1). BDNF has been linked to a variety of neurological and psychological conditions such as Huntington’s disease, Alzheimer’s disease, depression and anxiety. Studies show that transgenic overexpression of the neurotrophin BDNF has an unexpected facilitatory effect on anxiety-like behavior, concomitant with increased spinogenesis in the basolateral amygdala (2). In addition, BDNF enhances the neurite outgrowth on immature astrocytes. A multifactorial strategy based on transplantation of neonatal astrocytes in the presence of additional BDNF may stimulate axon regrowth after experimental injury to the central nervous system (3). The precursor pro-BDNF is 27-34 kDa before proteolytic cleavage, whereas the mature form of BDNF is 14 kDa.|
MAPK signaling pathway
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Western blot - BDNF antibody [EP1293]; All lanes : Anti-BDNF antibody [EP1293] at 1/1000 dilution.Lane 1 : C6 cell lysate.Lane 2 : Fetal brain lysate.Lane 3 : Human hippocampus lysate.Lane 4 : Recombinant BDNF protein.Lysates/proteins at 10 µg per lane.Predicted band size : 28 kDa.
Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EP1293]; TA307522 staining BDNF in human lung tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, permeabilized using 0.1% Saponin/PBS, blocked with 4% serum for 30 minutes at 25Â°C and then incubated with TA307522 at a 1/100 dilution for 14 hours at 4Â°C. The secondary used was a Texas Red conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - BDNF antibody [EP1293]; Immunofluorescent staining of PC12 cells using TA307522 at a dilution of 1/100.