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Anti-BDNF Antibody EP1293
Also for BDNF (NM_001709)
|A synthetic peptide corresponding to residues in human BDNF was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000 - 1:10000; ICC/IF: 1:100 - 1:250; IHC-Fr: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,IHC-P or IP.
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Western blot - BDNF antibody [EP1293]; All lanes : Anti-BDNF antibody [EP1293] at 1/1000 dilution.Lane 1 : C6 cell lysate.Lane 2 : Fetal brain lysate.Lane 3 : Human hippocampus lysate.Lane 4 : Recombinant BDNF protein.Lysates/proteins at 10 ug per lane.Predicted band size : 28 kDa.
Immunohistochemistry (Frozen sections) - Anti-BDNF antibody [EP1293]; TA307522 staining BDNF in human lung tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, permeabilized using 0.1% Saponin/PBS, blocked with 4% serum for 30 minutes at 25Â°C and then incubated with TA307522 at a 1/100 dilution for 14 hours at 4Â°C. The secondary used was a Texas Red conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - BDNF antibody [EP1293]; Immunofluorescent staining of PC12 cells using TA307522 at a dilution of 1/100.