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Anti-NR4A1 Antibody EPR3209
Also for NR4A1 (NM_002135)
|A synthetic peptide corresponding to residues near the N-terminus of human Nur77 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens nuclear receptor subfamily 4, group A, member 1 (NR4A1), transcript variant 1|
|GFRP1; HMR; N10; NAK-1; NGFIB; NP10; NUR77; TR3|
|Nur77 (TR3, NGFI-B) is an orphan nuclear receptor belonging to the steroid/thyroid hormone receptor superfamily with multiple cell functions relating to apoptosis, cell differentiation, and proliferation. Nur77 expression is rapidly induced by several growth factors, mitogens, and stress factors (1). With its central DNA binding domain, Nur77 binds to specific DNA elements and regulates the transcription of the target gene (2). While Nur77 activities are shown to be specific to its sub-cellular location, the phospholyation on Nur77 by various kinases (e.g. RSK, MSK) might affect its transactivation activity and its localization (3, 4). Nur77 localized in the nucleus functions as an oncogenic survival factor and promotes cell growth. However, in mitochondria, it functions as an apoptosis factor after binding to Bcl-2 and triggering cytochrome c release. Nur77 has been linked to apoptosis of immature T cell and T cell hybridoma. In tumors, it is involved in both apoptosis and proliferation of cancer cells (4).|
MAPK signaling pathway
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Western blot - NUR77 antibody [EPR3209]; All lanes : Anti-NUR77 antibody [EPR3209] at 1/1000 dilution.Lane 1 : SH-SY5Y cell lysate.Lane 2 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 64 kDa.Observed band size : 64 kDa.
Flow Cytometry - NUR77 antibody [EPR3209]; Flow cytometric analysis of permeabilized HepG2 cells using TA307475 at 1/10 (red) or a rabbit IgG in (green negative control).