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Home Antibody All anti-PDPK1 antibodies

Anti-PDPK1 PHOSPHO Antibody EPR336(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307427
  • Rabbit monoclonal antibody against PDK1 Phospho (pS241)(clone EPR336(2)) (Phospho-Specific)
100ul 325 3-7 Days
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WB(1)
IHC(1)
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Also for PDPK1 (NM_031268)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Serine 241 of human PDK1 was used as an immunogen. This antibody only detects PDK1 phosphorylated at Serine 241.
Clone NameEPR336(2) IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
Predicted MW Explanation 63 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant 2
Alternative NamePDK1; PDPK2; PRO0461
Database LinkNP_112558
Entrez Gene 5170 Human
Entrez Gene 18607 Mouse
Entrez Gene 81745 Rat
Function3-Phosphoinositide-dependent protein kinase 1 (PDK1) is a serine/threonine protein kinase known to phosphorylate and activate various signal transduction pathways: Akt/PKB (Thr308 and Ser473), p70 S6 kinase, RSK, PKA, PKC, and SGK. Upon stimulation of receptor tyrosine kinases, PI3K becomes activated and generates the phospholipid second messengers, PtdIns(3,4,5)P3 and PtdIns(3,4)P2, which in turn mediate the phosphorylation and activation of PDK1 targets through diverse mechanisms (1, 2). Unlike other phosphorylation sites on PDK1, only phosphorylation on the activation loop at Ser241 is necessary for PDK1 activity; a single mutation to Ala abolishes its activity (3). PDK1 can also autophosphorylate Ser241, leading to its own activation (3).
Related PathwayProtein KinaseDruggable Genome PPAR signaling pathwaymTOR signaling pathwayFocal adhesionInsulin signaling pathwayEndometrial cancerProstate cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PDPK1 (phospho S241) antibody [EPR336(2)]; All lanes : Anti-PDPK1 (phospho S241) antibody [EPR336(2)] at 1/1000 dilution.Lane 1 : 293T cell lysate .Lane 2 : 293T cell lysate treated with Lambda Phosphatase.Lysates/proteins at 10 ug per lane.Secondary.HRP-labeled goat anti-rabbit at 1/2000 dilution.Predicted band size : 63 kDa.Observed band size : 60 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDPK1 (phospho S241) antibody [EPR336(2)]; TA307427, at a 1/100 dilution, staining PDPK1 (phospho S241) in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

 

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