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Anti-PAX2 Antibody EP3251


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against PAX2 (clone EP3251 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418301) , 20ug Explanation
100ul $325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human PAX2 was used as an immunogen.
Clone NameEP3251 IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, FC Suggested DilutionsWB: 1:5000 - 1:10000; FC: 1:50; IHC-P: 1:1000 - 1:2500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens paired box 2 (PAX2), transcript variant c
Alternative NameFSGS7; PAPRS
Database LinkNP_003979
Entrez Gene 5076 Human
Entrez Gene 18504 Mouse
FunctionPAX2 is a transcription factor that plays a key role during renal development and angiogenesis and through the Akt-survival pathway (1). PAX2 is also expressed in normal renal medulla where it is regulated by the normally high and variable NaCl concentration, and it protects renal medullary cells from high NaCl concentration-induced apoptosis (2). Recent reports show that PAX2 also plays a role in breast cancer by competing with the ER co-activator AIB-1/SRC-3 for the binding and regulation of ERBB2 transcription (3). PAX2 is expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system (4).
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Pax2 antibody [EP3251]; Anti-Pax2 antibody [EP3251] at 1/10000 dilution + fetal kidney lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 45 kDa.Observed band size : 45 kDa.
Other-Anti-Pax2 antibody [EP3251](TA307329); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Pax2 antibody [EP3251]; TA307329 at 1/1000 dilution staining Pax2 in human fetal kidney (image A), human normal kidney (image B) and human renal cell carcinoma (image C) by Immunohistochemistry, Paraffin-embedded tissues.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251]; Immunohistochemical analysis of Human malignant melanoma tissue, staining Pax2 with TA307329. Antigen retrieval was performed via heat mediation in a citrate buffer (pH 6). Sections were blocked with 0.1% Triton X-100/PBS containing 1% BSA and 10% horse serum for 1 hour. Samples were incubated with primary antibody overnight at 4°C. A Cy3?-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Flow Cytometry-Pax2 antibody [EP3251](TA307329); Overlay histogram showing HEK293 cells stained with TA307329 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.


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