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Anti-ACTN4 Antibody EPR2533(2)
Also for ACTN4 (NM_004924)
|A synthetic peptide corresponding to residues on the N-terminus in human Alpha-Actinin 4 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens actinin, alpha 4 (ACTN4)|
|ACTININ-4; FSGS; FSGS1|
|Alpha-actinin is a ubiquitously expressed cytoskeletal protein found in numerous actin structures. It consists of an N-terminal actin-binding domain, a central rod domain, and a C-terminal domain; the protein functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinin 4 is a non-muscle isoform version of alpha-actinin that is concentrated in the cytoplasm (2). It plays a role in cell motility, invasion, and metastasis of cancer cells (3). Alpha-actinin 4 is commonly expressed in filopodia, lamellipodia, stress fibers, and sites of adhesion (4).|
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Western blot - alpha Actinin 4 antibody [EPR2533(2)]; All lanes : Anti-alpha Actinin 4 antibody [EPR2533(2)] at 1/1000 dilution.Lane 1 : Fetal skeletal muscle lysate.Lane 2 : A431 cell lysate.Lane 3 : MCF-7 cell lysate.Lane 4 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 105 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha Actinin 4 antibody [EPR2533(2)]; ab108198, at 1/100 dilution, staining alpha Actinin 4 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - alpha Actinin 4 antibody [EPR2533(2)]; ab108198, at 1/100 dilution, staining alpha Actinin 4 in HeLa cells by Immunofluorescence.