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Anti-Cebpb Antibody E299
Also for Cebpb (NM_005194)
|A synthetic peptide corresponding to residues in C-terminus of rat C/EBP b was used as immunogen. The antibody can recognize three C/EBPb isoform (i.e. LAP*, LAP, and LIP).|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||EMSA: Use at an assay dependent concentration; WB: 1:1000; ICC/IF: 1:50 - 1:100; FC: 1:1000; IP: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for IHC-P.
|Homo sapiens CCAAT/enhancer binding protein (C/EBP), beta (CEBPB), transcript variant 1|
|C/EBP-beta; CRP2; IL6DBP; LAP; LIP; NF-IL6; TCF5|
|CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (a,ß,?,d,e, ? ) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). A number of serine targets of PKA have been identified in C/EBP-ß, suggesting its activities are post-translationally regulated by cAMP (2). Even though, it has been show that C/EBP ß occurs predominantly as a heterodimer (3), C/EBP ß & ? readily heterodimerize with each other as well as with C/EBP a (4).|
EGFR1 Signaling Pathway
Senescence and Autophagy
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Western blot - CEBPB antibody [E299]; Anti-CEBP Beta antibody [E299] at 1/1000 dilution + PC12 cell lysate.Predicted band size : 36 kDa.
Western blot - CEBP Beta antibody [E299]; All lanes : Anti-CEBP Beta antibody [E299] at 1/500 dilution.Lane 1 : 3T3 Cells.Lane 2 : MCF7 Cells.Lane 3 : PC12 Cells.Lane 4 : Rat Spleen Lysate.Lane 5 : Rat Kidney Lysate.Lane 6 : Rat Heart Lysate.Lane 7 : Rat Brain Lysate.Lane 8 : Mouse Spleen Lysate.Lane 9 : Mouse Kidney Lysate.Lane 10 : Mouse Heart Lysate.Lane 11 : Mouse Brain Lysate.Predicted band size : 36 kDa.
Immunocytochemistry/ Immunofluorescence - CEBPB antibody [E299]; Ab32358, at a 1/50 dilution, staining CEBPB in Hela cells by Immunofluorescence.
Flow Cytometry - Anti-CEBP Beta antibody [E299]; Overlay histogram showing HeLa cells stained with ab32358 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.