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Anti-PDGFRA PHOSPHO Antibody EP2478
Also for PDGFRA (NM_006206)
|A phospho specific peptide corresponding to residues surrounding Tyrosine 720 of human PDGF Receptor alpha was used as an immunogen. This antibody only detects PDGF Receptor alpha phosphorylated at Tyrosine 720.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; FC: 1:500 - 1:1000
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for ICC,IHC-P or IP.
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Western blot - Anti-PDGF Receptor alpha (phospho Y720) antibody [EP2478]; All lanes : Anti-PDGF Receptor alpha (phospho Y720) antibody [EP2478] at 1/1000 dilution.Lane 1 : NIH 3T3 cell lysate, untreated.Lane 2 : NIH 3T3 cell lysate, treated with Pervanadate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 122 kDa.Observed band size : 190 kDa .
Flow Cytometry - Anti-PDGF Receptor alpha (phospho Y720) antibody [EP2478]; Flow Cytometry analysis of permeabilized NIH/3T3 cells labelling PDGF Receptor alpha (phospho Y720) with TA307172.Green - untreated, Red - pervanadate-treated, Blue - preincubated with phospho-PDGF Receptor alpha (phospho Y720) peptide, Orange - preincubated with non-phospho-PDGR Receptor alpha peptide.