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Anti-POU2AF1 Antibody EPR3299


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against OCA-B (clone EPR3299 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416779) , 20ug Explanation
100ul $325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human OCA-B was used as an immunogen.
Clone NameEPR3299 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsFC: 1:100, IP: 1:50, WB: 1:2,000 - 10,000,
Predicted MW Explanation 28 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens POU class 2 associating factor 1 (POU2AF1)
Alternative NameBOB1; OBF-1; OBF1; OCAB
Database LinkNP_006226
Entrez Gene 5450 Human
FunctionOCA-B is a lymphoid-specific transcriptional coactivator which interacts with the transcription factors Oct-1 and Oct-2 (1). It has been shown to be critical for development of a normal immune response, where it mediates octamer-dependent transcriptional activity in B lymphocytes. It is also critically involved in the formation of germinal centers in secondary lymphoid organs. OCA-B levels have been observed to be massively upregulated in germinal center B cells, as compared with resting B cells. SIAH1 and SIAH2, known regulators of protein stability, have been identified as two related OCA-B interacting proteins. SIAH1 has been suggested as part of a novel regulatory loop controlling the level of OCA-B protein in B cells. B-cell receptor cross-linking results in a degradation of OCA-B, which further results in a reduction of transcriptional activation of OCA-B-dependent reporters (2, 3).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - BOB1 antibody [EPR3299]; All lanes : Anti-BOB1 antibody [EPR3299] at 1/2000 dilution.Lane 1 : Ramos cell lysate.Lane 2 : Daudi cell lysate.Lane 3 : NAMALWA cell lysate.Lane 4 : Raji cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 28 kDa.


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