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Anti-POU2AF1 Antibody EPR3299
Also for POU2AF1 (NM_006235)
|A synthetic peptide corresponding to residues in human OCA-B was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||FC: 1:100, IP: 1:50, WB: 1:2,000 - 10,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens POU class 2 associating factor 1 (POU2AF1)|
|BOB1; OBF-1; OBF1; OCAB|
|OCA-B is a lymphoid-specific transcriptional coactivator which interacts with the transcription factors Oct-1 and Oct-2 (1). It has been shown to be critical for development of a normal immune response, where it mediates octamer-dependent transcriptional activity in B lymphocytes. It is also critically involved in the formation of germinal centers in secondary lymphoid organs. OCA-B levels have been observed to be massively upregulated in germinal center B cells, as compared with resting B cells. SIAH1 and SIAH2, known regulators of protein stability, have been identified as two related OCA-B interacting proteins. SIAH1 has been suggested as part of a novel regulatory loop controlling the level of OCA-B protein in B cells. B-cell receptor cross-linking results in a degradation of OCA-B, which further results in a reduction of transcriptional activation of OCA-B-dependent reporters (2, 3). |
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Western blot - BOB1 antibody [EPR3299]; All lanes : Anti-BOB1 antibody [EPR3299] at 1/2000 dilution.Lane 1 : Ramos cell lysate.Lane 2 : Daudi cell lysate.Lane 3 : NAMALWA cell lysate.Lane 4 : Raji cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 28 kDa.