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Anti-LEF1 Antibody EP2030Y
Also for LEF1 (NM_016269)
|A synthetic peptide corresponding to residues in human LEF-1/TCF-1 alpha was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||ICC/IF: 1:100 - 1:250; WB: 1:25000 - 1:50000; FC: 1:20; IHC-P: Use at an assay dependent dilution
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1|
|LEF-1; TCF10; TCF1ALPHA; TCF7L3|
|The human T cell-specific transcription factor TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) C alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions. TCF-1 alpha is currently unique among the newly emerging family of DNA-binding regulatory proteins that share the HMG motif in that it is a highly tissue-specific RNA polymerase II transcription factor (1). TCF-1 alpha participates in the regulation of TCR alpha enhancer by facilitating the assembly of multiple proteins into a higher order nucleoprotein complex. The function of LEF-1 is dependent, in part, on the HMG domain that induces a sharp bend in the DNA helix, and on an activation domain that stimulates transcription only in a specific context of other enhancer-binding proteins (2). Constitutive activation of the Wnt signaling pathway is a root cause of many colon cancers. Activation of this pathway is caused by genetic mutations that stabilize the beta-catenin protein, allowing it to accumulate in the nucleus and form complexes with any member of the lymphoid enhancer factor and T-cell factor family of transcription factors (referred to collectively as LEF/TCFs) to activate transcription of target genes (3). |
Delta-Notch Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - LEF1 antibody [EP2030Y]; Anti-LEF1 antibody [EP2030Y] at 1/500000 dilution + Jurkat cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 44 kDa.Observed band size : 55 kDa .
Immunohistochemistry (Paraffin-embedded sections) - LEF1 antibody [EP2030Y]; Ab53293 (1:50) staining human LEF1 in human colon adenocarcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Flow Cytometry - LEF1 antibody [EP2030Y]; Flow cytometric analysis of permeabilized Jurkat cells using ab53293