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Anti-INA Antibody EP676Y


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against NF66(clone EP676Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC409964) , 20ug Explanation
100ul $325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the tail domain of human NF-66 was used as immunogen.
Clone NameEP676Y IsotypeIgG
Species ReactivityMouse, Rat, Human (Does not react with: Apteronotus leptorhynchus) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsFC: 1:25, ICC: 1:250-500, IHC: 1:250-500, IP: 1:50, WB: 1:10,000-50,000,
Predicted MW Explanation 66 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Apteronotus leptorhynchus

Reference Data

Target NameHomo sapiens internexin neuronal intermediate filament protein, alpha (INA)
Alternative NameNEF5; NF-66; TXBP-1
Database LinkNP_116116
Entrez Gene 9118 Human
Entrez Gene 226180 Mouse
Entrez Gene 24503 Rat
FunctionNeurofilaments (NF) are cytoplasmic intermediate filaments (IFs) found in neurons. NF-66, also known as a-internexin, is a member of the IF family. Classified as a type IV IF, NF-66 is expressed by most neurons and has been shown to co-assemble with other NF triple proteins (light, medium and heavy NF) (1-2). In the adult brain, NF-66 is expressed at relatively low levels compared to the other NF proteins (3). NF-66 as also been shown to assemble into 10mm diameter IF in vitro.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - alpha Internexin antibody [EP676Y]; Anti-alpha Internexin antibody [EP676Y] at 1/10000 dilution + SHSY5Y cell lysate at 10 ug.Predicted band size : 66 kDa.Observed band size : 60 kDa .
Immunohistochemistry (Paraffin-embedded sections) - alpha Internexin antibody [EP676Y]; Positive staining of alpha Internexin on paraffin-embedded human brain tissue (A) using TA307027 diluited 1:250. Negative staining of paraffin-embedded human colon (B), Kidney (C) and uterus (D) tissues using TA307027 under the same conditions.
Immunofluorescence - alpha Internexin antibody [EP676Y]; Immunofluorescent staining of alpha Internexin on PC 12 cells using TA307027 diluted 1:250.


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