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Anti-EGFR PHOSPHO Antibody E124
Also for EGFR (NM_005228)
|A synthetic phospho-peptide corresponding to residues surrounding Tyr1173 of human EGF receptor was used as immunogen. The antibody detects EGFR phosphorylated on Tyrosine 1173 of the mature human isoform 1 (corresponding to Y1197 from the precursor form|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC: 1:25-50, IHC: 1:25-50, IP: 1:10, WB: 1:1,000-10,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Does not react with Mouse, Rat
|Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1|
|ERBB; ERBB1; HER1; mENA; PIG61|
|Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding (3). Once activated, EGFR phosphotyrosines Y-1068 and Y-1173 mediate the binding of Grb2 to the EGFR. Addtionally, phosphotyrosine Y-1173 is a major binding site for SHC on the EGFR (4).|
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - Anti-EGFR (phospho Y1173) antibody [E124]; All lanes : Anti-EGFR (phospho Y1173) antibody [E124] at 1/1000 dilution.Lane 1 : A431.Lane 2 : A431 + EGF cell lysates.Predicted band size : 170 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1173) antibody [E124]; TA306974 (at 1/50) staining (A) untreated and (B) Phosphatase-treated skin carcinoma tissue by IHC-P.
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124]; Immunofluorescent staining of untreated A431 cells using TA306974.
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124]; Immunofluorescent staining of Phosphatase-treated A431 cells using TA306974.