Home Antibody All anti-BUB1 antibodies
Also for BUB1 (NM_004336)
|Bub1 antibody was raised against a 19 amino acid peptide from near the amino terminus of human Bub1.|
||ELISA, WB: 2 - 4 ug/ml, ICC: 10 ug/ml, IF: 20 ug/ml
|PBS containing 0.02% sodium azide.|
|Affinity chromatography purified via peptide column
|Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant 1|
|BUB1A; BUB1L; hBUB1|
Entrez Gene 699 Human
Entrez Gene 12235 Mouse
|Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.|
|Protein KinaseDruggable Genome Cell cycleOocyte meiosisProgesterone-mediated oocyte maturation|
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Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 ug/mL.
Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 ug/mL.