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Anti-TEC Antibody Y398
|A synthetic peptide corresponding to residues in the PH domain of human Tec was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:2000 - 1:5000; IHC-P: Use at an assay dependent concentration; ICC: 1:100 - 1:250; FC: 1:30 - 1:100; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Homo sapiens tec protein tyrosine kinase (TEC)|
|The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. Tec family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. Tec kinase is an integral component of T cell signaling and has a distinct role in T cell activation. This gene may be associated with myelodysplastic syndrome. [provided by RefSeq]. |
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Western blot - Tec antibody [Y398]; Anti-Tec antibody [Y398] at 1/10000 dilution + Jurkat cell lysate.Predicted band size : 73 kDa.Observed band size : 66 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Tec antibody [Y398]; ab32368, at a 1/50 dilution,staining human ovary carcinoma by immunohistochemistry, Paraffin embedded tissue.
Flow Cytometry - Anti-Tec antibody [Y398]; Overlay histogram showing Jurkat cells stained with ab32368 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.