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Anti-SPI1 Antibody EPR3159Y
Also for SPI1 (NM_001080547)
|A synthetic peptide corresponding to residues on the C terminus of transcription factor PU.1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000; FC: 1:30; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for IP.
|Homo sapiens spleen focus forming virus (SFFV) proviral integration oncogene (SPI1), transcript variant 1|
|OF; PU.1; SFPI1; SPI-1; SPI-A|
|This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
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Western blot - PU.1/Spi1 antibody [EPR3159Y]; All lanes : Anti-PU.1/Spi1 antibody [EPR3159Y] at 1/1000 dilution.Lane 1 : U937 cell lysates.Lane 2 : TF-1 cell lysates.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 31 kDa.Observed band size : 42 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PU.1/Spi1 antibody [EPR3159Y]; TA303699, at 1/100 dilution, staining PU.1/Spi1 in paraffin-embedded human tonsil tissue by immunohistochemistry.