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Anti-SGK1 Antibody Y238
Also for SGK1 (NM_005627)
|A synthetic peptide corresponding to residues of human SGK was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:500; IHC-P: Use at an assay dependent dilution; FC: 1:30; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for ICC.
|Homo sapiens serum/glucocorticoid regulated kinase 1 (SGK1), transcript variant 1|
|This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]. |
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Western blot - SGK1 antibody [Y238]; All lanes : Anti-SGK1 antibody [Y238] at 1/500 dilution.Lane 1 : A431 cell lysate.Lane 2 : NIH3T3 cell lysate.Predicted band size : 49 kDa.Observed band size : 57 kDa .
Immunohistochemistry (Paraffin-embedded sections) - SGK1 antibody [Y238]; Ab32374, at a 1/50 dilution, staining SGK1 in paraffin embedded human kidney carcinoma tissue sections by Immunohistochemistry.
Flow Cytometry-Anti-SGK1 antibody [Y238](ab32374); Overlay histogram showing HEK293 cells stained with ab32374 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.