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Anti-CSNK2A1 Antibody EP1963Y
Also for CSNK2A1 (NM_177560)
|A synthetic peptide corresponding to residues near the N-terminal of human CKII protein was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:500 - 1:1000; FC: 1:20 - 1:50; IHC-P: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC or IP.
|Homo sapiens casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 3|
|CK2A1; CKII; CSNK2A3|
|Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq]. |
Wnt Signaling Pathway
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Western blot - CKII alpha antibody [EP1963Y]; Anti-CKII alpha antibody [EP1963Y] at 1/1000 dilution + HeLa cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 45 kDa.Observed band size : 45 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CKII alpha antibody [EP1963Y]; Immunohistochemical analysis of CKII alpha in paraffin embedded human breast carcinoma tissue using ab76040 at a 1/50 dilution.
Flow Cytometry - Anti-CKII alpha antibody [EP1963Y]; Overlay histogram showing Jurkat cells stained with ab76040 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.