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Home Antibody All anti-CSNK2A1 antibodies

Anti-CSNK2A1 Antibody EP1963Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303470
  • Rabbit monoclonal antibody against Casein Kinase II (alpha subunit) (EP1963Y )
100ul 325
$292
In Stock
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WB(1)
IHC(1)
FC(1)
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Also for CSNK2A1 (NM_177560)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminal of human CKII protein was used as an immunogen.
Clone NameEP1963Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:500 - 1:1000; FC: 1:20 - 1:50; IHC-P: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC or IP.

Reference Data

Target NameHomo sapiens casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 3
Alternative NameCK2A1; CKII; CSNK2A3
Database LinkNP_808228
FunctionCasein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq].
Related Pathway
Adherens junction
Wnt Signaling Pathway

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WB Image
Western blot - CKII alpha antibody [EP1963Y]; Anti-CKII alpha antibody [EP1963Y] at 1/1000 dilution + HeLa cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 45 kDa.Observed band size : 45 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CKII alpha antibody [EP1963Y]; Immunohistochemical analysis of CKII alpha in paraffin embedded human breast carcinoma tissue using TA303470 at a 1/50 dilution.
FC Image
Flow Cytometry - Anti-CKII alpha antibody [EP1963Y]; Overlay histogram showing Jurkat cells stained with TA303470 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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