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Home Antibody All anti-CDC6 antibodies

Anti-CDC6 PHOSPHO Antibody EPR759Y


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against CDC6 (Clone EPR759Y) (Phospho-specific)
100ul 325 3-7 Days
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OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding serine 54 of human Cdc6 was used as an immunogen. The antibody only detects Cdc6 phosphorylated on Serine 54.
Clone NameEPR759Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:1000 - 1:2000; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens cell division cycle 6 (CDC6)
Alternative NameCDC18L; HsCDC18; HsCDC6
Database LinkNP_001245
Entrez Gene 990 Human
FunctionThe Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle (1). Cdc6p and cdc18+ are unstable proteins that are essential and rate limiting for the initiation of DNA replication in Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively. They participate in checkpoint controls that ensure DNA replication is completed before mitosis is initiated (2). It has been concluded that expression of Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells (3). Cdc6 is believed to be phosphorylated at Serine 54 during S phase and functions as a chromatin-bound signal that prevents reformation of prereplication complexes (4).
Related Pathway Cell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Cdc6 (phospho S54) antibody [EPR759Y]; All lanes : Anti-Cdc6 (phospho S54) antibody [EPR759Y] at 1/2000 dilution.Lane 1 : Raji cell lysate.Lane 2 : Raji + FBS cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 63 kDa.Observed band size : 63 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - Cdc6 (phospho S54) antibody [EPR759Y]; Immunofluorescent staining of Cdc6 in HeLa cells using TA301267 at 1/100 dilution.


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