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Home Antibody All anti-PRKCB1 antibodies

Anti-PRKCB1 PHOSPHO Antibody EP1902Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301115
  • Rabbit Monoclonal Antibody against PRKCB1 (Clone EP1902Y) (Phospho-specific)
100ul 325 3-7 Days
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WB(1)
IHC(1)
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Also for PRKCB1 (NM_002738)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding serine 660 of human PKC?II was used as an immunogen. This antibody detects PKC?II phosphorylated at serine 660.
Clone NameEP1902Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsIHC-P: 1:100 - 1:250; WB: 1:50000 - 1:200000; ICC: 1:50 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens protein kinase C, beta (PRKCB), transcript variant 2
Alternative NamePKC-beta; PKCB; PRKCB1; PRKCB2
Database LinkNP_002729
FunctionPKC?II is a member of the serine/threonine protein kinase C (PKC) family (1) that includes 10 closely related isozymes classified as the conventional, the novel and the atypical. The conventional PKC?II is implicated in diverse cellular process including secretion, proliferation, differentiation, apoptosis, permeability, migration & hypertrophy (2-3). PKC?II structure consists of a C-terminal catalytic region, containing an activator-binding domain and a Ca2+-binding domain, and a regulatory domain. It has been shown that PKC?II must first be processed by three distinct phosphorylations before it is competent to respond to second messengers. Specifically, the threonine at position 500 (T500) on the activation loop, and T641 and S660 on the carboxyl terminus of protein kinase C beta II are phosphorylated in vivo. T500 and S660 are selectively dephosphorylated in vitro by protein phosphatase 2A to yield an enzyme that is still capable of lipid-dependent activation, whereas all three residues are dephosphorylated by protein phosphatase 1 to yield an inactive enzyme. (4).
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
MAPK signaling pathway
Melanogenesis
TGF Beta Signaling Pathway
Wnt Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PKC beta 2 (phospho S660) antibody [EP1902Y]; All lanes : Anti-PKC beta 2 (phospho S660) antibody [EP1902Y] at 1/100000 dilution.Lane 1 : Jurkat cell lysates, untreated.Lane 2 : Jurkat cell lysates, treated with AP.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 77 kDa.Observed band size : 77 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC beta 2 (phospho S660) antibody [EP1902Y]; TA301115, at a 1/100 dilution, staining PKC beta 2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.

 

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