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Anti-PRKCB1 PHOSPHO Antibody EP1902Y
Also for PRKCB1 (NM_002738)
|A phospho specific peptide corresponding to residues surrounding serine 660 of human PKC?II was used as an immunogen. This antibody detects PKC?II phosphorylated at serine 660.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||IHC-P: 1:100 - 1:250; WB: 1:50000 - 1:200000; ICC: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens protein kinase C, beta (PRKCB), transcript variant 2|
|PKC-beta; PKCB; PRKCB1; PRKCB2|
|PKC?II is a member of the serine/threonine protein kinase C (PKC) family (1) that includes 10 closely related isozymes classified as the conventional, the novel and the atypical. The conventional PKC?II is implicated in diverse cellular process including secretion, proliferation, differentiation, apoptosis, permeability, migration & hypertrophy (2-3). PKC?II structure consists of a C-terminal catalytic region, containing an activator-binding domain and a Ca2+-binding domain, and a regulatory domain. It has been shown that PKC?II must first be processed by three distinct phosphorylations before it is competent to respond to second messengers. Specifically, the threonine at position 500 (T500) on the activation loop, and T641 and S660 on the carboxyl terminus of protein kinase C beta II are phosphorylated in vivo. T500 and S660 are selectively dephosphorylated in vitro by protein phosphatase 2A to yield an enzyme that is still capable of lipid-dependent activation, whereas all three residues are dephosphorylated by protein phosphatase 1 to yield an inactive enzyme. (4). |
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Western blot - PKC beta 2 (phospho S660) antibody [EP1902Y]; All lanes : Anti-PKC beta 2 (phospho S660) antibody [EP1902Y] at 1/100000 dilution.Lane 1 : Jurkat cell lysates, untreated.Lane 2 : Jurkat cell lysates, treated with AP.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 77 kDa.Observed band size : 77 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC beta 2 (phospho S660) antibody [EP1902Y]; TA301115, at a 1/100 dilution, staining PKC beta 2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.