OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-TCF7L2 antibodies

Anti-TCF7L2 Antibody EP2033Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301000
  • Rabbit Monoclonal Antibody against TCF7L2 (Clone EP2033Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC403074) , 20ug
100ul $325 In Stock
Add to Shopping Cart
WB(1)
IHC(1)
IF(1)
FC(1)
spacer
Also for TCF7L2 (NM_030756)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human TCF-4 was used as an immunogen.
Clone NameEP2033Y IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:25000 - 1:100000; IP: 1:50; IHC-P: 1:100 - 1:250; FC: 1:50; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens transcription factor 7-like 2 (T-cell specific, HMG-box) (TCF7L2), transcript variant 2
Alternative NameTCF-4; TCF4
Database LinkNP_110383
FunctionTCF4 (HMG box transcription factor 4) is a member of the lymphoid enhancer factor family of transcription factors. Essential during development, along with beta catenin and Bcl 9, TCF4 participates in the Wnt signaling thru transactivation of downstreams genes (1). Commonly found in intestinal epithelium, TCF4 is required in the maintenance of crypt stem cell compartments of small intestines (2). Mutation in TCF4 has implicated in colorectal cancer and high expression of TCF4 variants has been linked in increased risk of type II diabetes (3).
Related Pathway
Adherens junction
Melanogenesis
Wnt Signaling Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - TCF7L2 antibody [EP2033Y]; Anti-TCF7L2 antibody [EP2033Y] at 1/100000 dilution + Jurkat cell lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 68 kDa.Observed band size : 68 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCF7L2 antibody [EP2033Y]; TA301000 at 1/100 dilution staining T cell Transcription Factor 4 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - TCF7L2 antibody [EP2033Y]; TA301000 at 1/100 dilution staining T cell Transcription Factor 4 in HeLa cells by Immunofluorescence.
FC Image
Flow Cytometry - Anti-TCF7L2 antibody [EP2033Y]; Overlay histogram showing Jurkat cells stained with TA301000 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title