OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-HOXB4 antibodies

Anti-HOXB4 Antibody EP1919Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300984
  • Rabbit Monoclonal Antibody against HOXB4 (Clone EP1919Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402972) , 20ug
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the middle region of human HoxB4 was used as immunogen.
Clone NameEP1919Y IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:40; ICC: 1:100 - 1:250; FC: 1:20; IHC-P: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens homeobox B4 (HOXB4)
Alternative NameHOX-2.6; HOX2; HOX2F
Database LinkNP_076920
FunctionHomebox transcription factors (Hox) are encoded by highly conserved developmental genes that are involved embryonic and early hematopoietic development. Specifically, Homeobox B4 (HoxB4) is a transcription factor encoded by HOXB4 gene and it is involved in the developmental regulation of specific positional identities on the anterior-posterior axis of cells. Like all members of HOX family, HoXB4 is abundantly expressed in primitive hematopoietic stem cell (HSC), but then decline with lineage-specific terminal differentiation (1). In HSC, stem cell self-renewal activity is regulated by USF1/2 binding to HoxB4, where binding possibly favors stem cell self-renewal instead of cell differentiation. Jun-B and Fra-1 has been linked as key mediators during cell proliferation and differentiation induced by HoxB4, which leads to increase expression of Cyclin D1 and G1 shortening (2).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - HOXB4 antibody [EP1919Y]; Anti-HOXB4 antibody [EP1919Y] at 1/500 dilution + K562 cell lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/1000 dilution.developed using the ECL technique.Predicted band size : 28 kDa.Observed band size : 34 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HOXB4 antibody [EP1919Y]; Immunohistochemical analysis of paraffin-embedded human placental tissue, using TA300984, at 1/100 dilution.
FC Image
Flow Cytometry-Anti-HOXB4 antibody [EP1919Y](TA300984); Overlay histogram showing K562 cells stained with TA300984 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title