OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-MAPK9 antibodies

Anti-MAPK9 Antibody EP1595Y

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300934 Rabbit Monoclonal Antibody against MAPK9 (Clone EP1595Y) 100ul $325 In Stock
LC400972 MAPK9 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
Add to Shopping Cart

spacer
WB(1)
IP(1)
IHC(1)
IF(1)
FC(1)
spacer
Also for MAPK9 (NM_002752)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human JNK2 was used as an immunogen.
Clone NameEP1595Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: Use a concentration of 5 ug/ml; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:40
Predicted MW Explanation 48 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase 9 (MAPK9), transcript variant JNK2-a2
Alternative NameJNK-55; JNK2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; p54a; p54aSAPK; PRKM9; SAPK; SAPK1a
Database LinkNP_002743
Entrez Gene 5601 Human
Entrez Gene 26420 Mouse
Entrez Gene 50658 Rat
FunctionJNK protein kinases are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group includes the 46-kDa isoform JNK1 and the 55-kDa protein kinase JNK2. The activities of both JNK isoforms are markedly increased by exposure of cells to UV radiation (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). JNK2 functions in a cell-type-specific and stimulus-dependent manner, being required for apoptosis of immature thymocytes induced by anti-CD3 antibody but not for apoptosis induced by anti-Fas antibody, UVC or dexamethasone. JNK2 is not required for activation-induced cell death of mature T cells (3).
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayWnt signaling pathwayFocal adhesionToll-like receptor signaling pathwayNOD-like receptor signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - JNK2 antibody [EP1595Y]; Anti-JNK2 antibody [EP1595Y] at 1/50000 dilution + HeLa cell lysate at 10 ug.Secondary.goat anti-rabbit-HRP at 1/1000 dilution.developed using the ECL technique.Predicted band size : 48 kDa.Observed band size : 54 kDa .Additional bands at : 46 kDa (possible isoform).
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - JNK2 antibody [EP1595Y]; TA300934 at 1/100 dilution staining JNK2 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
spacer
ICC/IF image of TA300934 stained HeLa cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was DyLight488 goat anti-rabbit IgG (H+L) used at 1:250. for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
spacer
JNK2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5ug of Rabbit monoclonal to JNK2 and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 48kDa; JNK2
spacer
Flow Cytometry-Anti-JNK2 antibody(TA300934); Overlay histogram showing HeLa cells stained with TA300934 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title