OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-CTNNA1 antibodies

Anti-CTNNA1 Antibody EP1793Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300925
  • Rabbit Monoclonal Antibody against CTNNA1 (Clone EP1793Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400708) , 20ug Explanation
100ul 325 3-7 Days
Add to Shopping Cart
WB(2)
IHC(1)
IF(1)
FC(1)
spacer
Also for CTNNA1 (NM_001903)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human a-1 catenin was used as an immunogen.
Clone NameEP1793Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:50000; IP: 1:50; ICC/IF: 1:100 - 1:250; FC: 1:100; IHC-P: Use at an assay dependent dilution
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens catenin (cadherin-associated protein), alpha 1, 102kDa (CTNNA1), transcript variant 1
Alternative NameCAP102
Database LinkNP_001894
Entrez Gene 1495 Human
Entrez Gene 12385 Mouse
Entrez Gene 307505 Rat
FunctionCadherin and catenin compose cell adhesion complex and are indispensable for tight cell-cell adhesion. Dysfunction of this adhesion complex causes dissociation of cancer cells from primary tumor nodules, thus possibly contributing to cancer invasion and metastasis (1). At least three proteins (alpha, beta, and gamma catenin) comprise the cytoplasmic domain of the cadherin cell-cell adhesion complex. Data, with the reported structure of other catenin genes, suggest that vinculin and alpha-catenin generate a superfamily of proteins mediating membrane-cytoskeletal associations (2). Presenilin-1 (PS1) overexpression in human kidney cells enhances cell-cell adhesion and data show that PS1 incorporates into the cadherin/catenin adhesion system and regulates cell-cell adhesion. PS1 concentrates at intercellular contacts in epithelial tissue; in brain, it forms complexes with both E- and N-cadherin and concentrates at synaptic adhesions (3).
Related PathwayDruggable Genome Adherens junctionTight junctionLeukocyte transendothelial migrationPathways in cancerEndometrial cancerArrhythmogenic right ventricular cardiomyopathy (ARVC)More Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - alpha 1 Catenin antibody [EP1793Y]; Anti-alpha 1 Catenin antibody [EP1793Y] at 1/50000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 100 kDa.Observed band size : 100 kDa.
WB Image
Western blot ; Anti-alpha 1 Catenin antibody [EP1793Y] at 1/50000 dilution + Human alpha 1 Catenin full length protein at 0.01 ug.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 1 minute
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - alpha 1 Catenin antibody [EP1793Y]; Ab51032 (1:100) staining human alpha 1 Catenin in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence-alpha 1 Catenin antibody [EP1793Y](TA300925); ICC/IF image of TA300925 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
FC Image
Flow Cytometry - alpha 1 Catenin antibody [EP1793Y]; Overlay histogram showing MCF7 cells stained with TA300925 (red line). The cells were fixed with 4% PFA (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title