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Anti-MET Antibody EP1454Y
|A synthetic peptide corresponding to residues near the N-terminus of human Met was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250; FC: 1:1000
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens met proto-oncogene (MET), transcript variant 2|
|AUTS9; c-Met; HGFR; RCCP2|
|Met is a receptor protein-tyrosine kinase (RPTK) for hepatocyte growth factor (HGF), which is a multifunctional cytokine controlling cell growth, morphogenesis, and motility. Met overexpression has been identified in a variety of human cancers (1). Met kinase domain possesses unique features that distinguish met from other members of the src family of protein tyrosine kinases. These results also demonstrate that the product of the activated met gene is a fusion protein and that the amino terminal end of this fusion protein exhibits homology to laminin B1 (2). Data suggest that RanBP9, functioning as an adaptor protein for the Met tyrosine kinase domain, can augment the HGF-Met signaling pathway and that RanBP9 overexpression may cause constitutive activation of the Ras signaling pathway (1). Hereditary papillary renal carcinoma (HPRC) is a recently recognized form of inherited kidney cancer Results suggest that missense mutations located in the MET proto-oncogene lead to constitutive activation of the Met protein and papillary renal carcinomas (3) |
Cytokine-cytokine receptor interaction
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Western blot - Met (c-Met) antibody [EP1454Y]; Anti-Met (c-Met) antibody [EP1454Y] at 1/2000 dilution + 293 cell lysate at 10 µg.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 156 kDa.Observed band size : 160 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Met (c-Met) antibody [EP1454Y]; Ab51067 (1/100-1/250) staining human Met (c-Met) in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Met (c-Met) antibody [EP1454Y](ab51067); ab51067 showing positive staining in Hepatocellular carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Met (c-Met) antibody [EP1454Y](ab51067); ab51067 showing positive staining in Thyroid gland carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Met (c-Met) antibody [EP1454Y](ab51067); ab51067 showing positive staining in Normal tonsil tissue.
Immunocytochemistry/ Immunofluorescence - Met (c-Met) antibody [EP1454Y]; ICC/IF image of ab51067 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry - Anti-Met (c-Met) antibody [EP1454Y]; Overlay histogram showing Jurkat cells stained with ab51067 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.