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Home Antibody All anti-MET antibodies

Anti-MET Antibody EP1454Y


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300895 Rabbit Monoclonal Antibody against MET (Clone EP1454Y) 100ul $325 In Stock
LC400094 MET HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Met was used as an immunogen.
Clone NameEP1454Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250; FC: 1:1000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens MET proto-oncogene, receptor tyrosine kinase (MET), transcript variant 2
Alternative NameAUTS9; c-Met; HGFR; RCCP2
Database LinkNP_000236
Entrez Gene 4233 Human
FunctionMet is a receptor protein-tyrosine kinase (RPTK) for hepatocyte growth factor (HGF), which is a multifunctional cytokine controlling cell growth, morphogenesis, and motility. Met overexpression has been identified in a variety of human cancers (1). Met kinase domain possesses unique features that distinguish met from other members of the src family of protein tyrosine kinases. These results also demonstrate that the product of the activated met gene is a fusion protein and that the amino terminal end of this fusion protein exhibits homology to laminin B1 (2). Data suggest that RanBP9, functioning as an adaptor protein for the Met tyrosine kinase domain, can augment the HGF-Met signaling pathway and that RanBP9 overexpression may cause constitutive activation of the Ras signaling pathway (1). Hereditary papillary renal carcinoma (HPRC) is a recently recognized form of inherited kidney cancer Results suggest that missense mutations located in the MET proto-oncogene lead to constitutive activation of the Met protein and papillary renal carcinomas (3)
Related PathwayProtein KinaseTransmembraneDruggable Genome Cytokine-cytokine receptor interactionEndocytosisAxon guidanceFocal adhesionAdherens junctionEpithelial cell signaling in Helicobacter pylori infectionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Met (c-Met) antibody [EP1454Y]; Anti-Met (c-Met) antibody [EP1454Y] at 1/2000 dilution + 293 cell lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 156 kDa.Observed band size : 160 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Met (c-Met) antibody [EP1454Y]; Ab51067 (1/100-1/250) staining human Met (c-Met) in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Met (c-Met) antibody [EP1454Y](TA300895); TA300895 showing positive staining in Hepatocellular carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Met (c-Met) antibody [EP1454Y](TA300895); TA300895 showing positive staining in Thyroid gland carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Met (c-Met) antibody [EP1454Y](TA300895); TA300895 showing positive staining in Normal tonsil tissue.
ICC/IF image of TA300895 stained HepG2 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry - Anti-Met (c-Met) antibody; Overlay histogram showing Jurkat cells stained with TA300895 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.


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