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Home Antibody All anti-MET antibodies

Anti-MET Antibody EP1454Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300895
  • Rabbit Monoclonal Antibody against MET (Clone EP1454Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400094) , 20ug
100ul 325 In Stock
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WB(1)
IHC(4)
IF(1)
FC(1)
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Also for MET (NM_000245)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Met was used as an immunogen.
Clone NameEP1454Y IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250; FC: 1:1000
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens met proto-oncogene (MET), transcript variant 2
Alternative NameAUTS9; c-Met; HGFR; RCCP2
Database LinkNP_000236
FunctionMet is a receptor protein-tyrosine kinase (RPTK) for hepatocyte growth factor (HGF), which is a multifunctional cytokine controlling cell growth, morphogenesis, and motility. Met overexpression has been identified in a variety of human cancers (1). Met kinase domain possesses unique features that distinguish met from other members of the src family of protein tyrosine kinases. These results also demonstrate that the product of the activated met gene is a fusion protein and that the amino terminal end of this fusion protein exhibits homology to laminin B1 (2). Data suggest that RanBP9, functioning as an adaptor protein for the Met tyrosine kinase domain, can augment the HGF-Met signaling pathway and that RanBP9 overexpression may cause constitutive activation of the Ras signaling pathway (1). Hereditary papillary renal carcinoma (HPRC) is a recently recognized form of inherited kidney cancer Results suggest that missense mutations located in the MET proto-oncogene lead to constitutive activation of the Met protein and papillary renal carcinomas (3)
Related Pathway
Adherens junction
Cytokine-cytokine receptor interaction
Focal Adhesion

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WB Image
Western blot - Met (c-Met) antibody [EP1454Y]; Anti-Met (c-Met) antibody [EP1454Y] at 1/2000 dilution + 293 cell lysate at 10 µg.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 156 kDa.Observed band size : 160 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Met (c-Met) antibody [EP1454Y]; Ab51067 (1/100-1/250) staining human Met (c-Met) in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Met (c-Met) antibody [EP1454Y](TA300895); TA300895 showing positive staining in Hepatocellular carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Met (c-Met) antibody [EP1454Y](TA300895); TA300895 showing positive staining in Thyroid gland carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Met (c-Met) antibody [EP1454Y](TA300895); TA300895 showing positive staining in Normal tonsil tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Met (c-Met) antibody [EP1454Y]; ICC/IF image of TA300895 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
FC Image
Flow Cytometry - Anti-Met (c-Met) antibody [EP1454Y]; Overlay histogram showing Jurkat cells stained with TA300895 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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