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Anti-MARK3 Antibody EPR633Y
Also for MARK3 (NM_002376)
|A synthetic peptide corresponding to residues near the C-terminus of human MARK3 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:2000; IP: 1:60; ICC: 1:50 - 1:100; FC: 1:30 - 1:1000; IHC-P: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens MAP/microtubule affinity-regulating kinase 3 (MARK3), transcript variant 3|
|CTAK1; KP78; Par-1a; PAR1A|
|MARK3 is a dual-specificity protein kinase that controls entry into mitosis by dephosphorylating Cdc2 on both threonine 14 and tyrosine 15. MARK3 is phosphorylated on serine 216 throughout interphase but not during mitosis. Serine 216 phosphorylation mediates the binding of 14-3-3 protein to MARK3, and MARK3/14-3-3 complexes are present throughout interphase but not during mitosis (1). Kinase suppressor of Ras (KSR) is a conserved component of the Ras pathway that interacts directly with MEK and MAPK. It has been shown that KSR1 translocates from the cytoplasm to the cell surface in response to growth factor treatment and that this process is regulated by MARK3 (2). MARK3 seems to be a positive regulator of the beta-catenin pathway and an inhibitor of the JNK pathway. These findings show that MARK3, a regulator of polarity, is also a modulator of Wnt-beta-catenin signalling, indicating a link between two important developmental pathways (3). |
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Western blot - Mark3 antibody [EPR633Y]; Anti-Mark3 antibody [EPR633Y] at 1/2000 dilution + HeLa cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Observed band size : 86 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Mark3 antibody [EPR633Y]; ab52626, at a 1/100 dilution, staining Human Mark3 in urinary bladder using Immunohistochemsitry, Paraffin embedded tissue.
Flow Cytometry - Anti-Mark3 antibody [EPR633Y]; Overlay histogram showing HeLa cells stained with ab52626 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.