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Anti-STK3 Antibody EP1466Y
Also for STK3 (NM_006281)
|A synthetic peptide corresponding to residues on the N-terminus of human Mst2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1: 10,000; IHC: 1:50 100; ICC: 1: 100 250; IP: 1: 100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens serine/threonine kinase 3 (STK3), transcript variant 1|
|Mammalian Sterile 20-Like 2 (Mst2) is most similar to the previously identified Mst1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in-vitro kinase assay indicates that Mst2 can phosphorylate an exogenous substrate, as well as itself, and phospho-amino-acid analysis indicates that it is a serine/threonine protein kinase (1) Mst2, a STE20-family member and purported Hpo ortholog, phosphorylates and activates both Lats1 and Lats2. Results indicate that Mst2-like kinases regulate Lats kinase activities in an evolutionarily conserved regulatory pathway (2). When stably expressed in HeLa cells, Mst highly sensitizes the cells to death receptor-mediated apoptosis by accelerating caspase-3 activation. These findings suggest that Mst1 and Mst2 play a role in apoptosis both upstream and downstream of caspase activation (3). |
MAPK signaling pathway
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Western blot - STK3 antibody [EP1466Y]; Anti-STK3 antibody [EP1466Y] at 1/10000 dilution + C6 cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 56 kDa.Observed band size : 56 kDa.
Immunohistochemistry (Paraffin-embedded sections) - STK3 antibody [EP1466Y]; Human lymphoma stained with ab52641 at 1/50 - 1/100 dilution