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Anti-STK4 Antibody EP1465Y
Also for STK4 (NM_006282)
|A synthetic peptide corresponding to residues near the N-terminus of human Mst1 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1: 10,000; IHC: 1: 100 250; ICC: 1: 100 250; FC: 1: 100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens serine/threonine kinase 4 (STK4)|
|KRS2; MST1; TIIAC; YSK3|
Entrez Gene 6789 Human
Entrez Gene 58231 Mouse
Entrez Gene 311622 Rat
|The human serine/threonine kinase, mammalian STE20-like kinase (MST), is considerably homologous to the budding yeast kinases, SPS1 and STE20, throughout their kinase domains. When stably expressed in HeLa cells, MST highly sensitizes the cells to death receptor-mediated apoptosis by accelerating caspase-3 activation. These findings suggest that MST1 and MST2 play a role in apoptosis both upstream and downstream of caspase activation (1). MST1 is cleaved and activated by caspases during apoptosis and is capable of inducing apoptotic morphological changes such as chromatin condensation upon overexpression (2). Mst1 can phosphorylate H2B at S14 in vitro and in vivo, and the onset of H2B S14 phosphorylation is dependent upon cleavage of Mst1 by caspase-3. These data reveal a histone modification that is uniquely associated with apoptotic chromatin in species ranging from frogs to humans and provide insights into a previously unrecognized physiological substrate for Mst1 kinase (3). |
|Protein KinaseDruggable Genome MAPK signaling pathwayPathways in cancerNon-small cell lung cancer|
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Western blot - MST1 antibody [EP1465Y]; Anti-MST1 antibody [EP1465Y] at 1/10000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 56 kDa.Observed band size : 59 kDa .
Immunohistochemistry (Paraffin-embedded sections) - MST1 antibody [EP1465Y]; Ab51134 (1:100) staining human MST1 in human urinary bladder tissue by immunohistochemistry using paraffin embedded tissue.