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Home Antibody All anti-ICAM1 antibodies

Anti-ICAM1 Antibody EP1442Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300830
  • Rabbit Monoclonal Antibody against ICAM1 (Clone EP1442Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400073) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)
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Also for ICAM1 (NM_000201)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human ICAM-1 was used as an immunogen.
Clone NameEP1442Y IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:2000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:100 - 1:250; FC: 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens intercellular adhesion molecule 1 (ICAM1)
Alternative NameBB2; CD54; P3.58
Database LinkNP_000192
Entrez Gene 3383 Human
FunctionAntigen-specific cell contacts in the immune system are strengthened by antigen-nonspecific interactions, mediated in part by lymphocyte-function associated (LFA) antigens. Recently, ICAM-1 (intercellular adhesion molecule-1) has been defined as a ligand for LFA-1. Monoclonal antibodies to ICAM-1 block T lymphocyte adhesion to fibroblasts and endothelial cells and disrupt the interaction between cytotoxic T cells and target cells. ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines (1). The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. However, ICAM-1 is also used as a receptor by the major group of human rhinoviruses and is a catalyst for the subsequent viral uncoating during cell entry (2). Monoclonal antibodies recognize a 95 kDa cell surface glycoprotein the major human rhinovirus receptor, ICAM-1 (3).
Related PathwayES Cell Differentiation/IPSTransmembraneDruggable Genome Cell adhesion molecules (CAMs)Natural killer cell mediated cytotoxicityLeukocyte transendothelial migrationViral myocarditis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ICAM1 antibody [EP1442Y]; Anti-ICAM1 antibody [EP1442Y] at 1/2000 dilution + Raji cell lysate at 10 ug.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 58 kDa.Observed band size : 89 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - ICAM1 antibody [EP1442Y]; TA300830, at a 1/50 dilution, staining Human ICAM1 in Tonsil using Immunohistochemistry, Paraffin embedded tissue.
FC Image
Flow Cytometry - Anti-ICAM1 antibody [EP1442Y]; Overlay histogram showing Raji cells stained with TA300830 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

 

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