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Anti-HMOX1 Antibody EP1391Y
Also for HMOX1 (NM_002133)
|A synthetic peptide corresponding to residues near the C-terminus of human HO-1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:2000; IP: 1:60; FC: 1:1000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens heme oxygenase (decycling) 1 (HMOX1)|
|bK286B10; HMOX1D; HO-1; HSP32|
|Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3). |
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Western blot - Heme Oxygenase 1 antibody [EP1391Y]; Anti-Heme Oxygenase 1 [EP1391Y] antibody at 1/2000 dilution + fetal liver lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 33 kDa.Observed band size : 33 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Heme Oxygenase 1 antibody [EP1391Y]; ab52947 at 1/100-1/250 dilution staining Heme Oxygenase 1 in human liver by Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Heme Oxygenase 1 antibody [EP1391Y]; ab52947 at 1/250-1/500 dilution staining Heme Oxygenase 1 in HeLa cells by Immunofluorescence.
Flow Cytometry - Anti-Heme Oxygenase 1 antibody [EP1391Y]; Overlay histogram showing HEK293 cells stained with ab52947 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.