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Anti-AR Antibody EP670Y

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SKU Description Amount Price Availability*  
TA300762
  • Rabbit Monoclonal Antibody against AR (Clone EP670Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC423281) , 20ug
100ul $325 In Stock
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WB(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human Androgen Receptor was used as an immunogen.
Clone NameEP670Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:10000; FC: 1:60
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC/IF,IHC-P or IP.

Reference Data

Target NameHomo sapiens androgen receptor (AR), transcript variant 2
Alternative NameAIS; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM
Database LinkNP_001011645
FunctionAndrogen receptor (AR) is a member of the steroid receptor superfamily that may require coactivators for proper or maximal transactivation. (1). The androgen receptor (AR) is essential for the growth of prostate cancer cells. It has been reported that tyrosine phosphorylation of AR is induced by growth factors and elevated in hormone-refractory prostate tumors. Data suggest that growth factors and their downstream tyrosine kinases, which are elevated during hormone-ablation therapy, can induce tyrosine phosphorylation of AR and such modification may be important for prostate tumor growth under androgen-depleted conditions (2). Cellular signaling occurs following androgen binding to the AR and translocation to the nucleus. This activated complex associates with androgen-responsive elements contained in the DNA sequence of target genes, affecting the transcriptional activity of these genes. Mutations may result in changes in the function or expression of AR protein, or of growth factors and their receptors under the influence of AR coactivators, and AR amplification could lead to activation of the receptor by reduced levels of androgens (3).
Related Pathway
TGF Beta Signaling Pathway

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WB Image
Western blot - Androgen Receptor antibody [EP670Y]; Anti-Androgen Receptor antibody [EP670Y] at 1/10000 dilution + LnCaP cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP antibody at 1/2000 dilution.Predicted band size : 99 kDa.Observed band size : 99 kDa.
FC Image
Flow Cytometry-Anti-Androgen Receptor antibody [EP670Y](TA300762); Overlay histogram showing PC3 cells stained with TA300762 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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