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Anti-AURKA Antibody EP1008Y
Also for AURKA (NM_198433)
|A synthetic peptide corresponding to residues near the C-terminus of human Aurora-A was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:50,000; IHC: 1: 100-250; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens aurora kinase A (AURKA), transcript variant 1|
|AIK; ARK1; AURA; AURORA2; BTAK; PPP1R47; STK15; STK6; STK7|
|Aurora-A is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process (1). Aurora-A is ubiquitously expressed and activated through an interaction with Ajuba and TPX2 during late G2 and mitotic phases. Activated form regulates the functions of centrosomes, spindles, and kinetochores required for normal mitotic progression. Initially localized at centrosome during interphase, Aurora-A is translocated to mitotic spindles in early mitotic phase and finally degraded after metaphase–anaphase transition (2). Overexpression of Aurora-A is seen various tumors, where an overexpression can induce abnormalities in G2 and Spindle checkpoints, and cytokinesis failure (3). |
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Western blot - Aurora A antibody [EP1008Y]; Anti-Aurora A antibody [EP1008Y] at 1/50000 dilution + HepG2 cell lysate at 10 µg.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 46 kDa.Observed band size : 46 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Aurora A antibody [EP1008Y]; ab52973, at a 1/100 dilution, staining Human Aurora-A in Cervical Carcinoma using Immunohistochemistry, Paraffin Embedded tissue. Bottom pannel is a larger magnification of the above image.