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Anti-CENPA Antibody EP800Y
Also for CENPA (NM_001809)
|A synthetic peptide corresponding to residues in the C-term of human CENP-A was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:20000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:250 - 1:500; FC: 1:80 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens centromere protein A (CENPA), transcript variant 1|
|Centromere protein A (CENP-A) is a 17 kD centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of a-satellite DNA into primary chromation structure (2). CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker (3). |
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Western blot - CENPA antibody [EP800Y]; Anti-CENPA antibody [EP800Y] at 1/20000 dilution + HeLa cell lysate (10ug/lane).Predicted band size : 16 kDa.Observed band size : 18 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y]; IHC image of ab45694 staining CENPA in Human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45694, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CENPA antibody [EP800Y](ab45694); ab45694 showing positive staining in Colonic adenocarcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CENPA antibody [EP800Y](ab45694); ab45694 showing positive staining in Lymphoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CENPA antibody [EP800Y](ab45694); ab45694 showing positive staining in Prostatic carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CENPA antibody [EP800Y](ab45694); ab45694 showing positive staining in Lung carcinoma tissue.
Immunocytochemistry/ Immunofluorescence - CENPA antibody [EP800Y]; Immunofluorescent staining of HeLa cells using ab45694 (1:250).
Flow Cytometry - Anti-CENPA antibody [EP800Y]; Overlay histogram showing HeLa cells stained with ab45694 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.