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Anti-ASNS Antibody EP282Y
Also for ASNS (NM_133436)
|A synthetic peptide corresponding to residues the C-terminus of human asparagine synthetase was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500-2,000; IHC: 1:50; ICC: 1:50-100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 1|
Entrez Gene 440 Human
Entrez Gene 27053 Mouse
Entrez Gene 25612 Rat
|Asparagine synthetase (AS) is a housekeeping enzyme responsible for the production of asparagine from aspartate and glutamine. Most mammalian cells express AS and regulate the level of activity in response to the concentration of asparagine in the medium. Certain tumor cells in contrast, exhibit little or no AS activity and rely on the surrounding medium as a source of exogenous asparagines (1). Therefore tumor cells may be selectively killed by a chemotherapeutic drug using asparaginase. This approach has been exploited in the treatment of certain cancers like childhood acute lymphoblastic leukemia (2).|
|Druggable Genome Alanine, aspartate and glutamate metabolismNitrogen metabolismMetabolic pathways|
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Western blot - Asparagine synthetase antibody [EP282Y]; Anti-Asparagine synthetase antibody [EP282Y] at 1/2000 dilution + K562 cell lysate at 10 ug.Predicted band size : 61 kDa.Observed band size : 60 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Asparagine synthetase antibody [EP282Y]; TA300650 at a 1:50 dilution staining Asparagine synthetase in human breast carcinoma tissue.
Immunohistochemistry (Paraffin-embedded sections) - Asparagine synthetase antibody [EP282Y]; TA300650 at a 1:50 dilution staining Asparagine synthetase in human muscle tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asparagine synthetase antibody [EP282Y]; Immunohistochemical analysis of Human stomach tissue, staining Asparagine synthetase with TA300650. Tissue was blocked with goat serum before incubating with primary antibody (1/50) overnight at room temperature. Staining was detected using DAB.