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Anti-ASNS Antibody EP282Y


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300650 Rabbit Monoclonal Antibody against ASNS (Clone EP282Y) 100ul $325 2 Weeks
LC403336 ASNS HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues the C-terminus of human asparagine synthetase was used as immunogen.
Clone NameEP282Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:500-2,000; IHC: 1:50; ICC: 1:50-100
Predicted MW Explanation 61 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 1
Alternative NameASNSD; TS11
Database LinkNP_597680
Entrez Gene 440 Human
Entrez Gene 27053 Mouse
Entrez Gene 25612 Rat
FunctionAsparagine synthetase (AS) is a housekeeping enzyme responsible for the production of asparagine from aspartate and glutamine. Most mammalian cells express AS and regulate the level of activity in response to the concentration of asparagine in the medium. Certain tumor cells in contrast, exhibit little or no AS activity and rely on the surrounding medium as a source of exogenous asparagines (1). Therefore tumor cells may be selectively killed by a chemotherapeutic drug using asparaginase. This approach has been exploited in the treatment of certain cancers like childhood acute lymphoblastic leukemia (2).
Related PathwayDruggable Genome Alanine, aspartate and glutamate metabolismNitrogen metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Asparagine synthetase antibody [EP282Y]; Anti-Asparagine synthetase antibody [EP282Y] at 1/2000 dilution + K562 cell lysate at 10 ug.Predicted band size : 61 kDa.Observed band size : 60 kDa .

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Asparagine synthetase antibody [EP282Y]; TA300650 at a 1:50 dilution staining Asparagine synthetase in human breast carcinoma tissue.

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Asparagine synthetase antibody [EP282Y]; TA300650 at a 1:50 dilution staining Asparagine synthetase in human muscle tissue.

IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asparagine synthetase antibody [EP282Y]; Immunohistochemical analysis of Human stomach tissue, staining Asparagine synthetase with TA300650. Tissue was blocked with goat serum before incubating with primary antibody (1/50) overnight at room temperature. Staining was detected using DAB.


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