OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-CD247 antibodies

Anti-CD247 Antibody EP286Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300633
  • Rabbit Monoclonal Antibody against CD247 (Clone EP286Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403667) , 20ug Explanation
100ul 325 In Stock
Add to Shopping Cart
spacer
WB(1)
FC(1)
spacer
Also for CD247 (NM_198053)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-terminus of human CD3 zeta was used as immunogen
Clone NameEP286Y IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:500; ICC: 1:250 - 1:500; FC: 1:20
Predicted MW Explanation 16 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens CD247 molecule (CD247), transcript variant 1
Alternative NameCD3-ZETA; CD3H; CD3Q; CD3Z; IMD25; T3Z; TCRZ
Database LinkNP_932170
Entrez Gene 919 Human
FunctionCD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR) (1). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C (1,2). CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways (3). Loss of the zeta chain results in the synthesis of unstable TCRs (4). A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases (5).
Related PathwayTransmembraneDruggable Genome Natural killer cell mediated cytotoxicityT cell receptor signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - CD3 zeta antibody [EP286Y]; Anti-CD3 zeta antibody [EP286Y] at 1/500 dilution + 10ug Jurkat cell lysate.Predicted band size : 16 kDa.Observed band size : 16 kDa.
FC Image
Flow Cytometry - Anti-CD3 zeta antibody [EP286Y]; Human peripheral blood lymphocytes stained with TA300633 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody for 30 min at 4°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1??/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy ??peripheral blood lymphocytes.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title