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Anti-CD247 Antibody EP286Y
Also for CD247 (NM_198053)
|A synthetic peptide corresponding to residues in the C-terminus of human CD3 zeta was used as immunogen|
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500; ICC: 1:250 - 1:500; FC: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens CD247 molecule (CD247), transcript variant 1|
|CD3-ZETA; CD3H; CD3Q; CD3Z; T3Z; TCRZ|
|CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR) (1). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C (1,2). CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways (3). Loss of the zeta chain results in the synthesis of unstable TCRs (4). A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases (5).|
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Western blot - CD3 zeta antibody [EP286Y]; Anti-CD3 zeta antibody [EP286Y] at 1/500 dilution + 10ug Jurkat cell lysate.Predicted band size : 16 kDa.Observed band size : 16 kDa.
Flow Cytometry - Anti-CD3 zeta antibody [EP286Y]; Human peripheral blood lymphocytes stained with TA300633 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22Â°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37Â°C. For experimentation, cells were treated with 50% methanol (-20Â°C) for 15 min at 4Â°C. Cells were then incubated with the antibody for 30 min at 4Â°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 4Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1??/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy ??peripheral blood lymphocytes.