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Anti-BBC3 Antibody EP512Y
Also for BBC3 (NM_014417)
|A synthetic peptide corresponding to residues in the C-term of human PUMA was used as immunogen. This antibody is predicted to detect both isoforms based on sequence analysis. The isoform B is ~18 kDa and isoform A is ~ 24kDa. Isoform B is primarily recog|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||FC: 1:30 - 1:100; WB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:250 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 4|
|JFY-1; JFY1; PUMA|
|PUMA, (p53 upregulated modulator of apoptosis), is activated by p53 in cells undergoing p53-induced apoptosis (1-2). Localized in the mitochondria PUMA induces cytochrome c release and activates the rapid induction of programmed cell death. PUMA interacts with Bcl2 (3), Bcl-XL, Bcl2-like-1 protein and promotes mitochondrial translocation and multimerization of BAX. PUMA also plays a role in mediating p53-induced cell death through the cytochrome c/APAF1-dependent pathway (2). Two isoform of PUMA are known; PUMA-alpha and PUMA-beta. |
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Western blot - PUMA antibody [EP512Y]; Anti-PUMA antibody [EP512Y] at 1/5000 dilution + K562 cell lysate.Predicted band size : 21 kDa.Observed band size : 18 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PUMA antibody [EP512Y]; ab33906 at a 1:100 dilution staining PUMA in human breast carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PUMA antibody [EP512Y]; ab33906 at a 1:100 dilution staining PUMA in human ovary carcinoma using Immunohistochemistry, Paraffin Embedded Tissue.
Immunocytochemistry/ Immunofluorescence - PUMA antibody [EP512Y]; ab33906 at a 1:250 dilution staining PUMA in Hela cells using Immunocytochemistry/Immunofluorescence.
Flow Cytometry - Anti-PUMA antibody [EP512Y]; Overlay histogram showing K562 cells stained with ab33906 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in K562 cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.