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Anti-RELA Antibody E379
Also for RELA (NM_021975)
|A synthetic peptide corresponding to residues near the C-terminus of human nuclear factor NF-kappa-B p65 subunit was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:50000 - 1:100000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; FC: 1:100; IP: 1:200
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 1|
|Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50, p52, p65 (RelA), c-Rel, and RelB. (2). The p65 subunit, similar to two others in the B family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein (3). Association with inhibitory proteins of the I?B family, retains NF-?B in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA (4). In addition to the nuclear translocation and DNA binding, NF-B’s transcriptional activity is also regulated by coactivators and corepressors in the nucleus. Interaction of p65 with coactivators; CREB-binding protein (Cat # 1496-1 & 1113-1) and p300, increases its transcriptional activity (5).|
Delta-Notch Signaling Pathway
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot - NFkB p65 antibody [E379]; Anti-NF-kB p65 antibody [E379] at 1/100000 dilution + HeLa cell lysate.Predicted band size : 65 kDa.Observed band size : 65 kDa.
Immunohistochemistry (Paraffin-embedded sections) - NFkB p65 antibody [E379]; Immunohistochemical analysis of paraffin-embedded human Breast carcinoma using anti-NF-kB p65 Rabbit Monoclonal Antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p65 antibody [E379]; Immunohistochemical analysis of colon sections from mice, staining NFkB p65 with ab32536. Antigen retrieval was performed by microwave heating in citrate buffer, pH 6. Sections were incubated overnight with primary antibody (1/250) and staining was detected using EXPOSE Rabbit specific HRP/DAB detection IHC kit.
Immunocytochemistry/ Immunofluorescence - NFkB p65 antibody [E379]; Immunofluorescent staining of HeLa cells using anti-NF-kB p65 Rabbit Monoclonal Antibody
Flow Cytometry - Anti-NFkB p65 antibody [E379]; Overlay histogram showing MCF-7 cells stained with ab32536 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.