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Home Antibody All anti-HRAS antibodies

Anti-HRAS Antibody Y132

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300461
  • Rabbit Monoclonal Antibody against HRAS (Clone Y132)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401645) , 20ug
100ul 325 In Stock
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WB(1)
IF(1)
FC(1)
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Also for HRAS (NM_005343)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-terminus of human H-Ras was used as an immunogen. The antibody does not cross-react with other Ras family member. Predicted to cross-react with chicken, based on sequence homology.
Clone NameY132 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:500; ICC/IF: 1:100 - 1:250; IP: 1:50; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified
Note Is unsuitable for IHC.

Reference Data

Target NameHomo sapiens Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 1
Alternative NameC-BAS/HAS; C-H-RAS; C-HA-RAS1; CTLO; H-RASIDX; HAMSV; HRAS1; p21ras; RASH1
Database LinkNP_005334
FunctionRas, a signal transducer, was first characterized as the transforming genes of Harvey & Kristen sarcoma virus (1). The Ras family (H-Ras, N-Ras, and K-Ras) regulates cell growth, differentiation and apoptosis (2). Switching from an active or resting state, Ras can either bind GTP or GDP respectively. In the triphosphate conformation, Ras will interact with GTPase activating protein (GAP) to increase its activity (3). Mutations in any of the three isoforms can convert these proteins into active oncogenes. Additionally, Ras mutations are found in 30 % of all human cancer (4).
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
MAPK signaling pathway
Melanogenesis
Senescence and Autophagy
TGF Beta Signaling Pathway

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WB Image
Western blot - GTPase HRAS antibody [Y132]; All lanes : Anti-GTPase HRAS antibody [Y132] at 1/500 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : PC12 cell lysate.Observed band size : 21 kDa .
IF Image
Immunocytochemistry/ Immunofluorescence - GTPase HRAS antibody [Y132]; Immunohistochemical staining of MCF7 cells using TA300461 at 1/100 dilution.
FC Image
Flow Cytometry - Anti-GTPase HRAS antibody [Y132]; Overlay histogram showing HeLa cells stained with TA300461 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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