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Anti-HRAS Antibody Y132
Also for HRAS (NM_005343)
|A synthetic peptide corresponding to residues in the C-terminus of human H-Ras was used as an immunogen. The antibody does not cross-react with other Ras family member. Predicted to cross-react with chicken, based on sequence homology.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB: 1:500; ICC/IF: 1:100 - 1:250; IP: 1:50; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Is unsuitable for IHC.
|Homo sapiens Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 1|
|C-BAS/HAS; C-H-RAS; C-HA-RAS1; CTLO; H-RASIDX; HAMSV; HRAS1; K-RAS; N-RAS; RASH1|
|Ras, a signal transducer, was first characterized as the transforming genes of Harvey & Kristen sarcoma virus (1). The Ras family (H-Ras, N-Ras, and K-Ras) regulates cell growth, differentiation and apoptosis (2). Switching from an active or resting state, Ras can either bind GTP or GDP respectively. In the triphosphate conformation, Ras will interact with GTPase activating protein (GAP) to increase its activity (3). Mutations in any of the three isoforms can convert these proteins into active oncogenes. Additionally, Ras mutations are found in 30 % of all human cancer (4). |
EGFR1 Signaling Pathway
MAPK signaling pathway
Senescence and Autophagy
TGF Beta Signaling Pathway
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Western blot - GTPase HRAS antibody [Y132]; All lanes : Anti-GTPase HRAS antibody [Y132] at 1/500 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : PC12 cell lysate.Observed band size : 21 kDa .
Immunocytochemistry/ Immunofluorescence - GTPase HRAS antibody [Y132]; Immunohistochemical staining of MCF7 cells using ab32417 at 1/100 dilution.
Flow Cytometry - Anti-GTPase HRAS antibody [Y132]; Overlay histogram showing HeLa cells stained with ab32417 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.