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Anti-HRAS Antibody Y132
Also for HRAS (NM_005343)
|A synthetic peptide corresponding to residues in the C-terminus of human H-Ras was used as an immunogen. The antibody does not cross-react with other Ras family member. Predicted to cross-react with chicken, based on sequence homology.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IF, FC
||WB: 1:500; ICC/IF: 1:100 - 1:250; IP: 1:50; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Is unsuitable for IHC.
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Western blot - GTPase HRAS antibody [Y132]; All lanes : Anti-GTPase HRAS antibody [Y132] at 1/500 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : PC12 cell lysate.Observed band size : 21 kDa .
Immunocytochemistry/ Immunofluorescence - GTPase HRAS antibody [Y132]; Immunohistochemical staining of MCF7 cells using TA300461 at 1/100 dilution.
Flow Cytometry - Anti-GTPase HRAS antibody [Y132]; Overlay histogram showing HeLa cells stained with TA300461 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.