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Anti-NBN Antibody Y112


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against NBN (Clone Y112)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419300) , 20ug Explanation
100ul $325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues near C-terminus of human Nibrin was used as immunogen
Clone NameY112 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:500; IP: 1:60; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:50; FC: 1:1000; ChIP/Chip: Use at an assay dependent concentration
Predicted MW Explanation 85 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction

Reference Data

Target NameHomo sapiens nibrin (NBN)
Alternative NameAT-V1; AT-V2; ATV; NBS; NBS1; P95
Database LinkNP_002476
Entrez Gene 4683 Human
Entrez Gene 27354 Mouse
Entrez Gene 85482 Rat
FunctionNibrin (also known as NBS1 or p95) contains 2 modules found in cell cycle check point; a forkhead-associated domain & a BRCA1 C-teminal repeat (1). In mammalian cells, Nibrin forms a complex with Mre11 & Rad50 (MRN). Nuclear localization of the MRN complex is relevant for chromosomal stability, meiotic recombination, DNA repair & telomere maintenance in eukaryotic cells(2-4). Mutation in Nibrin & Mre11 causes respectively two related disorders, Nijmegen breakage syndrome (NBS) & Ataxia-Telangiectasia- like disorder (ATLD).
Related PathwayDruggable Genome Homologous recombination

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - p95 NBS1 antibody [Y112]; Anti-p95 NBS1 antibody [Y112] - ChIP Grade at 1/500 dilution + HeLa cell lysate.Predicted band size : 85 kDa.Observed band size : 100 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - p95 NBS1 antibody [Y112]; Immunohistochemical analysis of p95 NBS1 expression in paraffin embedded human skin carcinoma tissue section, using 1/50 TA300448.
IF Image
Immunocytochemistry/ Immunofluorescence - p95 NBS1 antibody [Y112]; Immunofluorescent analysis of p95 NBS1 in HeLa cell culture, using 1/50 TA300448.
FC Image
Flow Cytometry - Anti-p95 NBS1 antibody [Y112] - ChIP Grade; Overlay histogram showing HeLa cells stained with TA300448 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


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