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Anti-KRT8 Antibody E431-2
Also for KRT8 (NM_002273)
|A synthetic phospho-peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8 was used as immunogen. The antibody only detects Keratin 8 phosphorylated on Serine 73.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||IHC-Fr: Use at an assay dependent concentration; WB: 1:10000; ICC/IF: 1:250; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt or IP.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 8 (phospho S73) antibody [E431-2]; IHC-P analysis (with antigen retrieval) of liver carcinoma using anti-Phospho-Keratin 8 diluted 1/500.
IHC of Mouse lung adenoma, staining Cytokeratin 8 (phospho S73) with TA300410.Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in PBS) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 (phospho S73) antibody [E431-2]; Immunofluorescent staining of A431 cells using anti-Phospho-Keratin 8 diluted 1/250.
Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 (phospho S73) antibody [E431-2]; Ab32579 staining cells from a human pancreatic cell line by ICC/IF. The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. Ab32579 was diluted 1/25 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor#174; 488 conjugated donkey anti-rabbit antibody was used as the secondary.