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Home Antibody All anti-KRT8 antibodies

Anti-KRT8 Antibody E431-2

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300410
  • Rabbit Monoclonal Antibody against KRT8 (Clone E431-2)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419425) , 20ug Explanation
100ul 325 In Stock
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IHC(2)
IF(2)
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Also for KRT8 (NM_002273)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8 was used as immunogen. The antibody only detects Keratin 8 phosphorylated on Serine 73.
Clone NameE431-2 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *IHC, IF Suggested DilutionsIHC-Fr: Use at an assay dependent concentration; WB: 1:10000; ICC/IF: 1:250; IHC-P: Use at an assay dependent dilution
Predicted MW Explanation 55 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens keratin 8 (KRT8), transcript variant 2
Alternative NameCARD2; CK-8; CK8; CYK8; K2C8; K8; KO
Database LinkNP_002264
Entrez Gene 3856 Human
Entrez Gene 16691 Mouse
Entrez Gene 25626 Rat
FunctionKeratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the ove
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 8 (phospho S73) antibody [E431-2]; IHC-P analysis (with antigen retrieval) of liver carcinoma using anti-Phospho-Keratin 8 diluted 1/500.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S73) antibody [E431-2]; Immunohistochemical analysis of Mouse lung adenoma, staining Cytokeratin 8 (phospho S73) with TA300410.Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in PBS) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 (phospho S73) antibody [E431-2]; Immunofluorescent staining of A431 cells using anti-Phospho-Keratin 8 diluted 1/250.
IF Image
Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 (phospho S73) antibody [E431-2]; Ab32579 staining cells from a human pancreatic cell line by ICC/IF. The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. Ab32579 was diluted 1/25 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor#174; 488 conjugated donkey anti-rabbit antibody was used as the secondary.

 

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