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Anti-CREB1 Antibody E113
Also for CREB1 (NM_134442)
|A synthetic phospho-peptide corresponding to residues surrounding Ser133 of human CREB was used as immunogen. The antibody only detects CREB phosphorylated on Serine 133. Predicted to cross-react with bovine, chicken, and zebra fish, based on sequence hom|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:500; IHC-P: 1:250; FC: 1:1000; IP: 1:40; ICC/IF: 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens cAMP responsive element binding protein 1 (CREB1), transcript variant B|
|CREB is a transcription factor protein that binds the cAMP response element (CRE), thereby stimulating transcription of this sequence. CREB plays a role in propagating numerous extracellular signals to the nuclues (1-3). CREB is activated by phosphorylation at the Ser133 position by various signaling pathways, and some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (1-3).|
EGFR1 Signaling Pathway
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Western blot - CREB (phospho S133) antibody [E113]; All lanes : Anti-CREB (phospho S133) antibody [E113] at 1/500 dilution.Lane 1 : A431 cells untreated.Lane 2 : A431 cells treated with forskolin.Predicted band size : 37 kDa.Observed band size : 46 kDa .
Western blot - Anti-CREB (phospho S133) antibody [E113]; .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 37 kDa.
Western blot - Anti-CREB (phospho S133) antibody [E113]; .Predicted band size : 37 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CREB (phospho S133) antibody [E113]; Ab32096, at a 1/250 dilution, staining CREB in paraffin embedded human thyroid gland adenocarcinoma tissue by Immunohistochemistry. .Image A: Cells are untreated. .Image B: Cells are treated with Phosphatase.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CREB (phospho S133) antibody [E113]; ab32096 staining CREB in Rat hippocampus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Sections were formalin-fixed, and subjected to antigen retrieval by autoclave prior to blocking with 8% milk for 30 minutes at 37°C. The primary antibody was diluted 1/100 with DAKO antibody diluent and incubated with the sample for 18 hours at 4°C. An LSAB-labeled Streptavidin-Biotin conjugated Goat polyclonal antibody was used undiluted as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - CREB (phospho S133) antibody [E113]; Ab32096, at a 1/250 dilution, staining CREB in A431 cells by Immunofluorescence. .Image A: Cells are untreated. .Image B: Cells are treated with Phosphatase.
Flow Cytometry - Anti-CREB (phospho S133) antibody [E113]; Overlay histogram showing A431 cells stained with ab32096 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.