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Home Antibody All anti-CASP3 antibodies

Anti-CASP3 Antibody E61

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300355
  • Rabbit Monoclonal Antibody against CASP3 (Clone E61)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403215) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for CASP3 (NM_032991)
cDNA Clone shRNA/siRNA Protein/Lysate Antibody Assay

OriGene Data

ImmunogenA synthetic peptide correscponding to N-terminal residues of Human Pro-Caspase-3 was used as immunogen. The antibody only recognizes the pro-form of Caspase-3. It does not react with the cleaved forms (active enzyme) of Caspase-3. This antibody is predict
Clone NameE61 IsotypeIgG
Species ReactivityHuman, Mouse ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsFC: 1:100; IP: 1:10; WB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Does not react with Rat

Reference Data

Target NameHomo sapiens caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta
Alternative NameCPP32; CPP32B; SCA-1
Database LinkNP_116786
FunctionCaspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).
Related Pathway
Apoptosis
MAPK signaling pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - pro Caspase 3 antibody [E61]; Anti-pro Caspase 3 antibody [E61] at 1/1000 dilution + Hela cell lysate.Observed band size : 35 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - pro Caspase 3 antibody [E61]; Immunohistochemical analysis of human paraffin-embedded cervical carcinoma tissue using TA300355 at 1/500 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - pro Caspase 3 antibody [E61]; Immunofluorescent staining of HeLa cells using TA300355 at 1/100 dilution.
FC Image
Flow Cytometry-Anti-pro Caspase 3 antibody [E61](TA300355); Overlay histogram showing Jurkat cells stained with TA300355 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (1µg/1x10^6 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

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