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Home Antibody All anti-BCL2L1 antibodies

Anti-BCL2L1 Antibody E18

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300337
  • Rabbit Monoclonal Antibody against BCL2L1 (Clone E18)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403363) , 20ug
100ul 325 In Stock
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WB(2)
IHC(2)
IF(1)
FC(1)
Assay(1)
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Also for BCL2L1 (NM_138578)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues between BH3 and BH3 of Human Bcl-x was used as the immunogen. This antibody should recognize Bcl-xL, Bcl-xS and Bcl-x(beta) as the immunogen sequence is common to them. The antibody does not cross-react with o
Clone NameE18 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF, FC Suggested DilutionsWB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100; FC: 1:100; IP: 1:10
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction

Reference Data

Target NameHomo sapiens BCL2-like 1 (BCL2L1), transcript variant 1
Alternative NameBcl-X; bcl-xL; BCL-XL/S; bcl-xS; BCL2L; BCLX; BCLXL; BCLXS; PPP1R52
Database LinkNP_612815
FunctionBcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1). Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2(2). It has been found that eterodimerization with Bax does not seem to be required for anti-apoptic activity (3). Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4). The Bcl-X(S) isoform promotes apoptosis.
Related Pathway
Apoptosis
Jak-STAT signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Bcl x antibody [E18]; Anti-Bcl-XL antibody [E18] at 1/1000 dilution + Jurkat cell lysate.Observed band size : 26 kDa .
WB Image
Western blot - BCL2L1 antibody [E18]; Anti-Bcl-XL antibody [E18] at 1/500 dilution + whole cell lysate prepared from a clinical sample of breast cancer cells at 20 ug.Secondary.Goat anti-rabbit IgG-HRP at 1/1000 dilution.developed using the ECL technique.Observed band size : 26 kDa .Exposure time : 15 minutes
Assay Image
Other-Anti-BCL2L1 antibody [E18](TA300337); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Bcl x antibody [E18]; Immunohistochemical analysis of human paraffin-embedded prostate carcinoma tissue using TA300337 at a 1/50 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L1 antibody [E18]; Immunohistochemistry of Human primary melanoma, staining BCL2L1 (red) with TA300337. Antigen retrieval was performed in EDTA/Tris buffer (pH 8) before being blocked with 10%NGS for one hour at room temperature. Samples were incubated with primary antibody (1/50) at room temperature for one hour. An AlexaFluor?-conjugated anti-rabbit IgG was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-BCL2L1 antibody [E18]; ICC/IF image of TA300337 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
FC Image
Flow Cytometry - Anti-BCL2L1 antibody [E18]; Overlay histogram showing DU145 cells stained with TA300337 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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