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Home Antibody All anti-BCL2L1 antibodies

Anti-BCL2L1 Antibody E18

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300337 Rabbit Monoclonal Antibody against BCL2L1 (Clone E18) 100ul $325 In Stock
LC403363 BCL2L1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)
IHC(2)
IF(1)
FC(1)
Assay(1)
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Also for BCL2L1 (NM_138578)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues between BH3 and BH3 of Human Bcl-x was used as the immunogen. This antibody should recognize Bcl-xL, Bcl-xS and Bcl-x(beta) as the immunogen sequence is common to them. The antibody does not cross-react with o
Clone NameE18 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, Assay, IHC, IF, FC Suggested DilutionsWB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100; FC: 1:100; IP: 1:10
Predicted MW Explanation 26 (Bcl-xL) / 19 (Bcl-xS) kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction

Reference Data

Target NameHomo sapiens BCL2-like 1 (BCL2L1), transcript variant 1
Alternative NameBcl-X; bcl-xL; BCL-XL/S; bcl-xS; BCL2L; BCLX; BCLXL; BCLXS; PPP1R52
Database LinkNP_612815
Entrez Gene 598 Human
Entrez Gene 12048 Mouse
Entrez Gene 24888 Rat
FunctionBcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1). Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2(2). It has been found that eterodimerization with Bax does not seem to be required for anti-apoptic activity (3). Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4). The Bcl-X(S) isoform promotes apoptosis.
Related PathwayES Cell Differentiation/IPSTransmembraneDruggable Genome ApoptosisJak-STAT signaling pathwayAmyotrophic lateral sclerosis (ALS)Pathways in cancerPancreatic cancerChronic myeloid leukemiaMore Pathways >>

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Bcl x antibody [E18]; Anti-Bcl-XL antibody [E18] at 1/1000 dilution + Jurkat cell lysate.Observed band size : 26 kDa .
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Western blot - BCL2L1 antibody [E18]; Anti-Bcl-XL antibody [E18] at 1/500 dilution + whole cell lysate prepared from a clinical sample of breast cancer cells at 20 ug.Secondary.Goat anti-rabbit IgG-HRP at 1/1000 dilution.developed using the ECL technique.Observed band size : 26 kDa .Exposure time : 15 minutes
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Other-Anti-BCL2L1 antibody [E18](TA300337); Equilibrium disassociation constant (KD)..
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Immunohistochemistry (Paraffin-embedded sections) - Bcl x antibody [E18]; Immunohistochemical analysis of human paraffin-embedded prostate carcinoma tissue using TA300337 at a 1/50 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCL2L1 antibody [E18]; Immunohistochemistry of Human primary melanoma, staining BCL2L1 (red) with TA300337. Antigen retrieval was performed in EDTA/Tris buffer (pH 8) before being blocked with 10%NGS for one hour at room temperature. Samples were incubated with primary antibody (1/50) at room temperature for one hour. An AlexaFluor?-conjugated anti-rabbit IgG was used as the secondary antibody.
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ICC/IF image of TA300337 stained HepG2 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was , DyLight 488 goat anti-rabbit IgG (H+L) used at 1:250. for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
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Flow Cytometry - Anti-BCL2L1 antibody; Overlay histogram showing DU145 cells stained with TA300337 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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