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Anti-PPP2CA Antibody E155
Also for PPP2CA (NM_002715)
| A synthetic phospho-peptide corresponding to residues surrounding Tyr307 of human PP2A was used as immunogen. The antibody only detects PP2A phosphorylated on Tyrosine 307. Predicted to cross-react with mouse, rat, bovine and pig, based on sequence homol|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:5000; IHC-P: Use at an assay dependent dilution; ICC: 1:50; IP: 1:100; ICC/IF: Use a concentration of 5 ug/ml
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt.
|Homo sapiens protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA)|
|PP2Ac; PP2CA; PP2Calpha; RP-C|
Entrez Gene 5515 Human
Entrez Gene 19052 Mouse
Entrez Gene 24672 Rat
|Protein phosphatase type 2A (PP2A) is a protein serine/threonine phosphatase that controls fundamental cellular processes, including transcription, translation, metabolism, cell growth, apoptosis, and varied other signal transduction pathways (1-3). PP2A is comprised of a core enzyme, which is made up of catalytic C and regulatory A (PR65) subunits, and a secondary regulatory B subunit. This B subunit contains four different protein families. In response to EGF or insulin, it has been shown that Src phosphorylates PP2A at Tyr307, thereby inactivating PP2A (4).|
|PhosphataseTranscription FactorsDruggable Genome Oocyte meiosisWnt signaling pathwayTGF-beta signaling pathwayTight junctionLong-term depression|
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Western blot - PP2A alpha (phospho Y307) antibody [E155]; All lanes : Anti-PP2A alpha (phospho Y307) antibody [E155] at 1/5000 dilution.Lane 1 : untreated A431 cell lysate.Lane 2 : EGF treated A431 cell lysate (the specific EGF concentration and incubation time is confidential information).Predicted band size : 35 kDa.Observed band size : 35 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha (phospho Y307) antibody [E155]; Immunohistochemical analysis of PP2A alpha catalitic subunit (phospho Y307) expression in paraffin-embedded human brain, using 1/50 TA300307.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-PP2A alpha (phospho Y307) antibody [E155](TA300307); TA300307 (2ug/ml) staining PP2A alpha (phospho Y307) in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of the islet of Langerhans.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence-PP2A alpha (phospho Y307) antibody [E155](TA300307); ICC/IF image of TA300307 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.